Effects of Ammonium on Intracellular pH in Rat Medullary Thick Ascending Limb: Mechanisms of Apical Membrane Transport
نویسنده
چکیده
Address correspondence to David W. Good, PhD, Division of Nephrology, 4.200John Seal}' Hospital E62, University of Texas Medical Branch, Galveston, Texas 77555-0562. j. GEN. PHYSIOL. 9 The Rockefeller University Press 9 0022-1295/94/05/0917/20 $2.00 Volume 103 May 1994 917-936 917 on F ebuary 3, 2013 jgp.rress.org D ow nladed fom Published May 1, 1994
منابع مشابه
Effects of ammonium on intracellular pH in rat medullary thick ascending limb: mechanisms of apical membrane NH4+ transport
UNLABELLED The renal medullary thick ascending limb (MTAL) actively reabsorbs ammonium ions. To examine the effects of NH4+ transport on intracellular pH (pHi) and the mechanisms of apical membrane NH4+ transport, MTALs from rats were isolated and perfused in vitro with 25 mM HCO3(-)-buffered solutions (pH 7.4). pHi was monitored using the fluorescent dye BCECF. In the absence of NH4+, the mean...
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